[Ebook Fungi Gardening] Growing Mushrooms The Easy Way.pdf

Growing Mushrooms the Easy Way

Home Mushroom Cultivation

with Hydrogen Peroxide

Volumes I + II

by R. Rush Wayne, Ph.D.

Nan's Nook : Archives : Misc Tek : Straw Tek : Sterilize Straw with Peroxide :

Volume II

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Growing Mushrooms the Easy Way

Home Mushroom Cultivation with Hydrogen Peroxide

R. Rush Wayne. Ph.D.

form or by any means without permission of the author.

ooops...

First published as Growing Mushrooms with Hydrogen Peroxide, December

1996

Third Edition revised November 1999

Visit the Updates Page of the Growing Mushrooms the Easy Way Web site

( http://members.aol.com/PeroxyMan/Updates.html )

for periodic corrections and updates to this manual, notes on sources of

supplies,

and news about the peroxide method.

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CONTENTS

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Introduction

Preliminaries

The Mushrooms

Hypsizygus ulmarius (Elm oyster)

Pleurotus eryngii (King oyster)

Hericium erinaceus (Lion’s Mane)

Agaricus subrufescens (Almond mushroom)

Lentinula edodes (Shiitake)

Pleurotus ostreatus (Oyster mushrooms)

Ganoderma lucidum (Reishi mushroom)

Coprinus comatus (Shaggy Mane)

Hypsizygus tessulatus (Shimeji)

Stropharia rugosa-annulata (King stropharia)

Equipment You Will Need

Specialized Supplies You May Need

The Basics on Peroxide

What peroxide does

Biological effect of peroxide

Advantages of using peroxide in mushroom culture

Contaminant resistance to peroxide

What peroxide does not do

The need for caution when exposing mycelium

Peroxide is not a sterilant at mushroom-growing concentrations

Safety and environmental considerations for hydrogen peroxide

Peroxide and the spirit of organic growing

Lack of effect of peroxide on substrate or mushroom cultures

Stability

In pure solution

At higher temperatures

In the presence of peroxide-decomposing enzymes

Guarding the purity of peroxide stock solution

Variations in peroxide concentration from commercial sources

Measuring peroxide concentration : Calculating how much peroxide solution

to use

Growing and Maintaining Agar Cultures

Preparing agar plates

MYA medium

Use the lowest effective concentration of peroxide in agar

No-pressure agar medium

Hazards of agar drips and importance of clean plates

Reusing peroxide agar medium

Acquiring mushroom cultures

Importance of starting with good strains

Cloning Mushrooms

Strain storage.

Distilled water method

Keep storage cultures peroxide-free

Inoculating and handling agar cultures

Cooling hot scalpels

Inoculating from storage cultures or peroxide-free medium

Preventing occult contamination with bottom inoculation: cleaning the

mycelium

Incubating inoculated plates and storing uninoculated plates

Making Mushroom Spawn

Economic advantage of making your own spawn

Advantages of sawdust-based spawn

"Ten minute" no-autoclave sawdust spawn

Nitrogen supplements compatible with Ten Minute Spawn

Importance of clean containers in making Ten Minute Spawn

Pressure-sterilized sawdust spawn

Grain spawn

Difficulties of grain spawn and pitfalls in spawn making

Spawn containers

Inoculating spawn

Agar chunk method

Use peroxide-adapted mycelium for spawn inoculation

Incubating and shaking the spawn

Liquid culture

Colonization of bulk substrate

Importance of choosing substrates lacking peroxide-decomposing enzymes

Pasteurizable substrates compatible with peroxide

Recipes for fruiting substrates

Wood chips and substrate density

Preparing supplemented sawdust with peroxide

Nitrogen supplements for bulk substrate

Calculating how much supplement to add

Measuring pH of substrate

Culture containers

Trash bags as culture containers

Excluding fungus gnats

Plastic buckets as an alternative to bags

Inoculating supplemented sawdust

Breaking up spawn for inoculation into fruiting substrates

Adding the spawn to the substrate

Growing mushrooms on straw

Hydrated lime soak method

Inoculating straw

Mushroom formation

General procedures for fruiting oyster-like mushrooms and Hericium

Protecting yourself from spores

Mushrooms needing a casing layer

Seasonal planning

Outdoor vs. indoor growing

Harvesting

Trouble shooting.

Conclusion

About the Author

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Introduction

Back to Contents

When I first took an interest in growing mushrooms, I checked out a well-

known book on mushroom cultivation from the library and eagerly read

through it. But my interest soon turned to general discouragement as I read

about all the equipment and procedures the book insisted were necessary to

grow mushrooms without getting the cultures contaminated. I would need a

sterile laboratory space with a laminar-flow hood fitted with electrostatic and

HEPA filters and an ultraviolet light. This space would need a sterile air-lock

entry way with a foot wash, and I would need to have special clothing to enter

it, so that I could wash down the floors with chlorine bleach every day. My

fruiting mushrooms would have to be grown in a separate building altogether,

so as to avoid getting spores into the sterile laboratory. These fruiting cultures

would have to be grown in specially designed plastic bags with microporous

filter patches attached, so that the mushroom mycelium could get oxygen

without letting mold spores or bacteria get in. Of course, I would need an

autoclave or at least a specially designed pressure cooker to sterilize the

media that went into these bags.

After considering these requirements briefly, I put aside the thought of

growing mushrooms. I wasn’t about to get all that equipment, and I figured I

probably wasn’t cut out for the job anyway. From what I could gather, my

house would be a death trap for mushroom cultures. Neither my wife nor I are

careful housekeepers. We have unabashed dust and clutter, and green and

white fuzzy things can be found in and outside the refrigerator. Although I was

skilled at sterile technique from my years as a graduate student in

biochemistry, I didn’t think that would save me from the legions of eager

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